Abstract: Excavation of the Harney Flats site (8-Hi-507), a Paleo-Indian site in Hillsborough County, Florida, resulted in the recovery of over one thousand unifacially and bifacially retouched stone tools. A primary objective in the analysis of these tools was to determine how they were used or functioned in various site activities. To facilitate the analysis of tool function, a series of use-wear experiments were conducted in which stone tools, similar to those recovered from 8-Hi-507, were manufactured by a contemporary flintknapper. These tools were then used to work a number of raw materials considered to be available to the aboriginal inhabitants of the site. The raw materials worked were bone, antler, wood, and hide. Experimental tools were used in a variety of scraping, sawing, and chopping tasks involving these raw materials. Use-wear traces such as tool edge and surface flaking, rounding, polish, and striation occurring on experimental tools were microscopically examined at magnifications to 99x for evidence of patterning indicative of the material worked and tool action employed. Results indicate that subtle differences in the character, intensity, and distribution of utilization damage observed on experimental tools can be used to establish the manner in which a tool was used and to identify the material worked, at least in terms of broad categories based on the relative hardness of the raw material. Experimentally derived data concerning utilization damage patterns were used in an analysis of a sample of the stone tools recovered from 8-Hi-507. This analysis was hindered by the patination and weathering of many of the archaeological specimens which served to obliterate, obscure, or, in some cases, mimic potential wear traces. Nevertheless, the experimentally derived data allowed for the functional interpretation of a number of tools recovered from the Harney Flats site.